Quant-iT PicoGreen dsDNA Assay Kit a. • Suitable for use in 96- and 384-well plate formats. The DeNovix dsDNA Broad Range Assay is ideal for measuring sample concentrations ranging from 0. 50 pg to 2 µg. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. 95) in the range of 10 pg till 100 ng for the salmon dsDNA as well as for the control dsDNA from the kit (λ dsDNA standard) (Fig. Data were expressed as mean ± standard deviation (SD) of n = 3 unless otherwise specified. P7589) was used to quantify DNA by fluorescence. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 99 to $1999. 5 ng/µL. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. Signal measurements of all samples were performed using the EnVision 2105 Multimode Plate Reader (PerkinElmer, Waltham, MA,. The PicoGreen reagent was discarded and the cells were rinsed with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) solution for 10 min. It must be completely liquified before use by allowing it to come to room temperature. • Specific for dsDNA in the presence of RNA. Bioz Stars score: 86/100, based on 1 PubMed citations. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. 3,201 3. The PicoGreen is sticking to the sides of the cuvette or assay plate. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. 99. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Add µL of the Quant-iT™ PicoGreen® dsDNA Reagent (detailed in Section ) to each sample. Catalog number: P7581. P11496). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Both fluorescent assays were performed with a Qubit fluorometer. ” J Pharm Biomed Anal. Rapidly detect as little as 0. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. 326,933 327K. Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which. 5 µL of each extracted sample was used for Picogreen analysis. (D–G) TLR stimulation was measured using a HEK293-TLR reporter. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. Q33120). quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. n = 3. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Bioz Stars score: 86/100, based on 1 PubMed citations. Technical note | Quant-iT and Qubit 1X dsDNA assaysthe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Blog May 11, 2021. Natural cures with herbs are. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). This protocol is a simplified and c. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for each cell line of interest. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. dsDNA만을 선택적으로 정량. samples as well as standard in the assay itself. Le réactif et les kits de quantification d’ADNdb PicoGreen sont parfaits pour les dosages PCR, les échantillons de biopuces, les dosages de lésions de l’ADN, les dosages d’activité enzymatique, la quantification d’ADN génomique, la mesure de l’ADNdb dans des mélanges complexes et la quantification d’ADN viral. NET formation quantification was performed using a MPO-DNA PicoGreen assay according to the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Assay kit, Invitrogen). 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. High linearity and sensitivity over a broad DNA range. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). PicoGreen dsDNA quantitation reagents are. The Quant-iT™ OliGreen™ ssDNA Assay Reagent enables researchers to quantify as little as 1 ng/mL oligonucleotide or ssDNA (200 pg in a 200 µL assay volume) with a. The assay is highly selective for double- stranded DNA over RNA, and in the. P11495. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Materials & Equipment Required But Not Provided • Pipettors - 5 L - 1200 L. 5. 3 Q32854 26. Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. Molecular Probes fluorescent and luminescent protein blot stains—Table 9. Measure concentration with a high-sensitivity assay (e. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. Add 5uL of PicoGreen reagent for every 1mL of buffer. DNA content was then determined using the Quant-iT PicoGreen dsDNA assay kit per manufacturer’s instructions. Approximate fluorescence excitation/emission maxima: 510/527 nm (see Figure 1, page 2) Quant-iT ™ dsDNA Broad-Range Assay Kit Catalog no. 2. Learn how to use the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit to quantitate double-stranded DNA (dsDNA) in solution with a fluorometer or a microplate reader. Quant-iT PicoGreen dsDNA Assay Kit a. Each Helixyte™ assay kit is specially formulated with a target-selective DNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Free dye. Showing 4 of 4. Quant-iT RiboGreen RNA Reagent The Quant-iT RiboGreen RNA Reagent is one of the most sensitive fluorescent dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. For a standard. • Specific for dsDNA in the presence of RNA. Showing 4 of 4. Prepare only the amount of 1:25 diluted PicoGreen required for that run. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Q33120) • Quant-iT dsDNA Assay Kit, broad range (Cat. The reagent must be completely thawed before using it by bringing it to room temperature. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. • Specific for dsDNA in the presence of RNA. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Figure 2. 8 List of Tables Molecular Probes amine-reactive dyes—Table 1. Rapidly detect as little as 0. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. 20–1,000 ng Qubit RNA BR Assay Kit 5–100 ng Qubit RNA HS Assay Kit Varioskan LUX Multimode Microplate Reader 2–1,000 ng Quant-iT dsDNA BR Assay Kit 0. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. Quantitation Range. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. One microgram of DNA was sheared using an M220. 线，利用这一曲线并根据经验检测样本的浓度。Qubit dsDNA HS Assay可用于测量DNA浓度在10pg/µL 至100ng/µL 之间 的样本。超过此浓度范围的样本必须经过稀释然后进行重新 测试，以确保它们的结果落在参考标准的线性范围内。Qubit dsDNA HS Assay具有高度的准确. 0, 20X c. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Table 2 summarizes results of the. Picogreen assays are compatible with most microplate readers and fluorometers. 005% Triton X-100, Tween-20, or Brij. Product Type. PicoGreen dsDNA quantitation reagents are. Qubit™ dsDNA Quantification Assay Kits. BR reagent (Component A) 1. Quantitation Range. 99. Rapidly detect as little as 0. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Quantifications using the UVette require half the amount ofCell viability was measured using a WST-8 assay (Cell Counting Kit-8, Dojindo). 99 to $1999. PicoGreen dsDNA quantitation reagents are. Picogreen assays are compatible with most microplate readers and fluorometers. o cDNA quantification using Quant-iT PicoGreen dsDNA Assay Kit (P7589, Life Technologies) o PCR Cycles used as indicated in NUM_PCR_CYCLES_cDNA and NUM_PCR_CYCLES_Nextera x C1_ADD_Reagents: o Randomer added to Lysis Reaction Mix, (3. Product Type. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Supplier: Invitrogen™ P11496. Figure 8. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. quant it picogreen dsdna assay kit reagents. Rapidly detect as little as 0. • Specific for dsDNA in the presence of RNA. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. 0 fluorometer according to the manufacturer’s protocols; a. 2–100 ng, the fluorescence signal is linear with DNA. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. Unit Size. a) 16. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Quant-iT PicoGreen dsDNA kit by Thermo Scientific Catalog #: P7589 2Prepare 1XTE buffer from 20X stock solution using nuclease-free water: will need 200 μl/well (for. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. 製品番号（カタログ番号）： P7581. 1X dsDNA assay (%) 100 17. 0 mL to 200 uL to fit a 96-well microplate format. Module in combination with PicoGreen® dye allows the direct quantitation of dsDNA in as little as 100 µL total volume. dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. Using 20x TE buffer make 1x solution. Reagent Search & Data Services Menu Reagent Search. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Suitable for use in 96- and 384-well plate formats. low-range. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). PicoGreen dsDNA quantitation reagents are. DNA. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. 0 ml 200x in DMSO b. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. 12. 1. This protocol is a simplified and This protocol is a simplified and condensed version of the full protocol from the manufacturer. 10 x 100 µL. Picogreen assays are compatible with most microplate readers and fluorometers. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Picogreen assays are compatible with most microplate readers and fluorometers. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Cloning, expression and proteins purification. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Not for use in diagnostic procedures. It must be completely liquified before use by allowing it to come to room temperature. Using this data as a starting point, the optimal conditions (e. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Rapidly detect as little as 0. Effect of the buffer dilution on the DNA measurement with the Quant-It PicoGreen dsDNA assay. 10. 2–100 ng of DNA. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. 10. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. 2023-11. Table 2 summarizes results of the. Picogreen assays are compatible with most microplate readers and fluorometers. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. 50 pg to 2 µg. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. dsDNA standards were incubated in cuvettes and measured using a Varian Cary Eclipse. Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes. Figure 2. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. PicoGreen®試薬アッセイは、蛍光を基にした迅速かつ簡単な手法により、dsDNAを正確に定量することができます。. • Suitable for use in 96- and 384-well plate formats. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 5mL. Louis, MI, USA). Bioz Stars score: 86/100, based on 1 PubMed citations. Description. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. DNA standard curve. Fluorescence was measured at. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. Serial dilutions were made to give appropriate cell, seeding numbers. Product Type. io |. Prepare the 1x TE buffer by diluting the 20x TE concentrate 1:20 by deionized water. 95 mL TE for the . 2. 1 Prepare a 2 μ g/mL stock solution of dsDNA in TE. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. Selectively detect dsDNA from a wide variety of sources, and even in presence of. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. PicoGreen dsDNA quantitation reagents are. Three kits covering a wide range of dsDNA concentrations. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. Three kits covering a wide range of dsDNA concentrations. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. 0. PicoGreen dsDNA quantitation reagents are. For duplicate-well droplet. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. 3. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. Quant-iT™ PicoGreen® dsDNA Assay KitLife TechnologiesCatalog #P7589 protocols. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. Quant Ittm Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Quantify nucleic acids and proteins using four user-selectable fluorescent. Alternatively, purified RCA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. 2009;49(4):997–1002. Reagents – The Quant-IT™ PicoGreen® kit was purchased from Thermo Fisher Scientific. Each sample was amplified in triplicate, and the triplicate amplicons were pooled and purified by gel extraction and quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, P7589). 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. Lambda DNA was used to generate the. The kit included 20X TE buffer (200 mM Tris-HCl, 20 mM EDTA, pH 7. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. Related applications: Nucleic Acid Quantitation. Revised: 10–June–2008 | MP 07581 Table 1. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. High tolerance for contaminants. 2 The input amount of linear dsDNA library is based on the available linear dsDNA (see Table 3). Quant It. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. 1. Picogreen assays are compatible with most microplate readers and fluorometers. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. PicoGreen dsDNA quantitation reagents are. Determination of DNA Yield. Natural cures with herbs are. Picogreen assays are compatible with most microplate readers and fluorometers. Unit Size. 22. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. This is Standard 1 which will have a final concentration of 1 ug/mL. 実験してみた – PicoGreenをプレートリーダーで使用してみた. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. Bioz Stars score: 99/100, based on 1 PubMed citations. The tubes were. Similar. Prepared DNA concentration of the samples was 250 ng/ mL. The DeNovix dsDNA High. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. This protocol is a simplified and condensed. The Qubit dsDNA BR Assay kit contains a highly selective enhancing reagent specific for quantifying dsDNA using volumes as low as 1 µL in a standard 96-well microplate format. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. USA) and quantity was measured using Qubit 2. Quant It Picogreen Dsdna Staining Kit, supplied by Thermo Fisher, used in various techniques. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. Picogreen assays are compatible with most microplate readers and fluorometers. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Fluorometric dsDNA Assay. 7. 反应次数. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Catalog Number. 5 µL of each extracted sample was used for Picogreen analysis. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. Buy from Supplier. 製品概要. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. • Suitable for use in 96- and 384-well plate formats. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. 文件. . Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Product Description. quant it picogreen dsdna kit - by Bioz Stars , 2023-09. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 500/525. Got it. Short-read sequencing was performed on an Illumina NextSeq 2000 platform (2x151bp. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). g. Buy from Supplier. PicoGreen dsDNA quantitation reagents are. Picogreen assays are compatible with most microplate readers and fluorometers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Figure 2. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. 2023. In addition, the concentration of DNA obtained from the 1860 tomato samples was measured fluorometrically using Quant-iT™ PicoGreen™ dsDNA Assay Kit (hereafter PicoGreen, Thermo Scientific, Waltham, MA, USA) and a 96-wells plate reader VICTOR3 1420 (PerkinElmer, Waltham, MA, USA) equipped with an excitation filter F485 and. PicoGreen dsDNA Assay Kit. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. (2012). UV흡광 결과보다 민감도 우수. 2. The accuracy index is defined in Table 2. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. A comparison of reagents for detecting and quantitating proteins in solution—Table 9. Quant It Picogreen Dsdna Quantitation Assay Kit, supplied by Thermo Fisher, used in various techniques. High Sensitivity and Broad Range dsDNA Quantitation Superior sensitivity and broad dynamic range A ccuBlue™ dsDNA quantitation kits offer ultra-sensitive and selective detection of dsDNA with minimal effects from common contaminants. The Rapidly detect as little as 0. Quant-iT™ PicoGreen ® dsDNA Assay Kit. Rapidly detect as little as 0. Touch devices users use touch & swipe gestures. Bioz Stars score: 86/100, based on 1 PubMed citations. 0 fluorometer and Quant-iT PicoGreen dsDNA. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all. 3. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. 86 / 100 stars. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. This kit contains. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 3. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されて. Bioz Stars score: 86/100, based on 1 PubMed citations. • Specific for dsDNA in the presence of RNA. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2.